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nupG [2018-05-30 17:46:20]
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nupG [2018-05-30 17:46:20]

purine nucleoside transporter
Locus
BSU39020
Isoelectric point
7.76
Molecular weight
43.56 kDa
Protein length
397 aa Sequence Blast
Gene length
1191 bp Sequence Blast
Function
purine uptake
Product
purine nucleoside transporter
Essential
no
Synonyms
yxjA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Transporters → Transporters/ other → Nucleotide/ nucleoside transporter
  • Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of purine nucleotides
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    4,005,752 → 4,006,945

    Phenotypes of a mutant

  • a nupN nupG double mutant is unable to utilize externally provided guanosine PubMed

    • The protein

    Protein family

  • SLC28A transporter family (according to Swiss-Prot)

    • Structure

  • 3TIJ (from Vibrio cholerae, 25% identity) PubMed

    • Localization

  • cell membrane PubMed

    • Expression and Regulation

    Operons

    Genes
    nupG

    Regulatory mechanism

  • G-box: termination, PubMed, in G-box

    • Regulation

  • expression activated by glucose (4.3 fold) PubMed
  • the G-box RNA is degraded by RNase Y PubMed
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    Biological materials

    Mutant

  • MGNA-B725 (yxjA::erm), available at the NBRP B. subtilis, Japan
  • CS219 (nupG::aphA3, available in Colin Harwood's and Jörg Stülke's labs) PubMed
  • BKE39020 (ΔnupG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATAGCAACTCCTATGA, downstream forward: _UP4_TAGAAACATTGAGCCATATC
  • BKK39020 (ΔnupG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATAGCAACTCCTATGA, downstream forward: _UP4_TAGAAACATTGAGCCATATC

    • References

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